The aim of present was to develop an accurate, precise, reproducible and economical UV spectrophotometric area under curve method for estimation of tramadol hydrochloride. This Area under curve of UV spectrum between 267 to 277 nm was validated as per ICH guideline Q2 (R1). The linearity for this method was found to be in the range of 60-160 μg/ml. The calibration curve was drawn by plotting graph between AUC and concentration. Coefficient of correlation was found to be 0.9967. The lower limit of detection and the limit of quantification were found to be 1.93 μg/ml and 5.84 μg/ml respectively. The percentage recovery was found to be 97.02±0.0681.
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